vimentin is a kind of type iiiinterfilament and has high structural homogenity with c-fos, c-jun and creb . vimentin also modulates translocation of some signal molecule . whether vimentin is a downstream event of plc-1 pathway and whether vimentin is an interchange site between plc-1 pathway and other signal pathways needs further confirmation by molecular biology analysis such as yeast two-hybrid system and western blot 實(shí)驗(yàn)結(jié)果顯示:經(jīng)過(guò)egf刺激24小時(shí)后,相對(duì)于野生型mef組,敲除型mef組的2一de圖譜中代表vimeniin的蛋白斑點(diǎn)消失,暗示缺失plc一y1基因可能直接或間接導(dǎo)致了vimentin的不表達(dá),vimentin是否是plc一1通路的下游事件,以及是否是plc一yl通路與其他信號(hào)通路的交叉點(diǎn),尚需結(jié)合其他方法進(jìn)行深入研究。